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Category:Utilities for macOS[Analysis of the biokinetic behavior of glycogen in the guinea pig small intestine by means of the analysis of the lactate-glycogenolysis complex. Part 2. Physiologic aspects].
After 5 days of dietary deprivation, the guinea pig small intestine exhibits signs of adaptation. The biokinetic behaviour of glycogen was analysed at this time by means of the lactate-glycogenolysis complex. Although the glycogen content increased by 50%, the total amount of lactate released into the intestinal lumen was reduced by 50% during 15 min of perfusion. During this same time span, lactate levels of 20 mM were maintained in the incubation medium, although the glycogenolysis process was completely blocked in the presence of the glycogen phosphorylase inhibitor levallorphan. Thus, it seems that the breakdown of glycogen within the intestine proceeds at a slower rate than its synthesis. The increase in glycogenolysis is mainly due to a decreased level of total glycolytic capacity within the intestinal cells. The lactate-glycogenolysis complex is a useful technique for the analysis of intestinal metabolism in animals which have adapted to dietary deprivation.Differential regulation of host cell factor (HCF) mRNA levels and hnRNP K expression during melanoma differentiation.
The developmentally regulated transcription factor microphthalmia-associated transcription factor (MITF) is required for terminal melanogenesis, and its expression is tightly linked to the differentiation program. HCF is the binding partner of MITF and is also important for melanocyte differentiation, but its expression is not linked to the differentiation program. We have used the human melanoma cell line, SK-Mel-28, and its doxycycline-inducible melanocyte lineage clone, K-Mel-2, to characterize the expression and regulation of hnRNP K and HCF during melanocyte differentiation. Doxycycline-induced K-Mel-2 cells, as well as hnRNP K-overexpressing SK-Mel-28 cells, exhibited enhanced tyrosinase activity, melanin synthesis, and melanosome aggregation in the presence of dbcAMP. In contrast, HCF levels increased only moderately in these doxycycline-treated cells. During melanin synthesis, MITF and tyrosinase 01e38acffe
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